Agrarian
Bulletin
of the Urals

Russian Journal of Agricultural Research

The publication is registered by the Ministry of the Russian Federation
for Affairs of the Press, Television and Radio Broadcasting and Mass Communication Media.
Registration certificate: PI number 77-12831 on May 31, 2002
Subscription index in catalog «Russian Press» - 16356
ISSN 1997 - 4868 (Print)

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Journal is included in the list of VAK (from 25.09.2017), No. 291

ISSN 2307-0005 (Online)
Key title: Agrarnyj vestnik Urala (Online)
Abbreviated key title: Agrar. vestn. Urala (Online)

Аграрный вестник Урала № 11 (165) 2017

Биология и биотехнологии

Слепцов Е. С. доктор ветеринарных наук, профессор Якутский научно-исследовательский институт сельского хозяйства

Винокуров Н.В. кандидат ветеринарных наук, старший научный сотрудник

Захарова О.И. аспирант Якутский научно-исследовательский институт сельского хозяйства

УДК:619:579.841.93:616-079.3

APPROBATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY WITH MONOCLONAL ANTIBODIES IN THE DIAGNOSIS OF BRUCELLOSIS OF REINDEER

 In the article results of approbation of ELISA basedon monoclonal antibodies in the diagnosis of brucellosis of reindeer conductedin the laboratory of reindeer breeding and traditional branches of the YakutScientific Research Institute of Agriculture and on the experimental base ofthe Russian Scientific Research Institute of Experimental Veterinary. Y. R.Kovalenko. Positive and questionable reactions with the help of agglutinationreaction (AR) + complement fixation reaction (CFR) complex, 29 samples weredetected – 8.2 % from the number of investigated, with the help of ELISA-35samples – 10 % (the ELISA test fully coincided with the readings of AR + CFR,in addition to AR + CFR, 6 samples – 1.7 % of those studied). In all 35 sampleswith positive and questionable reactions in AR + CFR and/or ELISA, a negativeresult was obtained in the immunodiffusion reaction (IR) with O-polysaccharideantigen, which indicates the absence of epizootically dangerous animals in thesurveyed herds. In ELISA, of the 25 test sera, a positive result of 8 sampleswas shown (optical density of 1.362 – 2.063), which indicates the completecompliance of the ELISA indicators with the indirect hemagglutination reaction(IHGR) and the diagnostic complex AR + CFR and the greater diagnosticsensitivity of ELISA compared to the indicated antibody detection to brucella.


Keywords:

brucellosis, infection process, immunity, strain, epizootic process, vaccine, reindeer.


References:

1. Vinokurov N. V., Sleptsov E. S. A study of the diagnostic efficacy of IHGR in brucellosis of reindeer // Veterinary Medicine and Feeding. 2007. № 6. P. 35.

2. Vinokurov N. V., Sleptsov E. S. A study of the diagnostic efficiency of the indirect hemagglutination reaction in case of brucellosis // Yakutsk Medical Journal. 2008. № 4. P. 72–73.

3. Vinokurov N. V., Evgrafov G. G., Sleptsov Е. S. Epizootology of brucellosis of reindeer in various zones of the Republic of Sakha (Yakutia) // Innovative developments of young scientists to the development of agro-industrial complex : proc. of the III International scient. and pract. conf. Stavropol, 2014. Vol. 2. № 7. P. 352–355.

4. Vinokurov N. V., Laishev K. A., Sleptsov E. S., Evgrafov G. G. Reactogenic properties and immunological reactivity of weakly-agglutinogenic vaccines from strains B.abortus 75/79-AB and 82 for reindeer // Messenger of St. Petersburg State Agrarian University. 2014. № 36. P. 79–81.

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9. Sleptsov E. S., Vinokurov N. V., Fedorov V. I., Evgrafov G. G. Immunological reactivity of reindeer organism after reimmunization by vaccines from strains B.abortus 82 and B.abortus 75/79-АВ // Agrarian Bulletin of the Urals. 2011. № 4. P. 27.

10. Sleptsov E. S., Iskandarov M. I., Vinokurov N. V., Evgrafov G. G., Evgrafova A. V. The results of bacteriological studies of deer crop strains B.suis 45 and B.suis 245 in the body of guinea pigs // Innovative development of young scientists – development of agro-industrial complex : proc. of intern. scient. and pract. conf. Stavropol, 2013. P. 256.

11. Sleptsov E. S., Vinokurov N. V., Ustinceva Y. Y., Malysheva I. A., Zakharova O. I. Immunogenicity of the vaccine from strains B.abortus 19 and 82, B.suis 61 for reindeer in different methods of administration // Agrarian Bulletin of the Urals. 2014. № 8. P. 21–22.

12. Sleptsov E. S., Iskandarov M. I., Vinokurov N. V., Evgrafov G. G., Evgrafova A. V. Preliminary results of studying the properties of cultures from strains B.suis 45 and B.suis 245 on laboratory animals // Bulletin of Buryat State Agricultural Academy named after V. R. Filippov. 2014. № 3. P. 28–31.

13. Sleptsov E. S., Iskandarov M. I., Vinokurov N. V., Evgrafov G. G., Evgrafova A. A. The results of bacteriological studies of “reindeer cultures” from strains B.suis 45 and B.suis 245 in the organism of guinea pigs // Innovative developments of young scientists to the development of agro-industrial complex : proc. of the II International scient. and pract. conf. Stavropol, 2013. Vol. 3. №6. P. 256–258.

14. Sleptsov E. S., Vinokurov N. V., Evgrafov G. G. Reactogenic, antigenic and immunogenic properties of the culture from pcs. B.suis 61 in experiments on guinea pigs // Agricultural Bulletin of the Urals. 2015. № 7. P. 32–35.

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