Аграрный вестник Урала № 03 (170) 2018Биология и биотехнологии
ANTIFUNGAL ACTIVITY OF FUSARIUM SPOROTRICHIOIDES ANTAGONISTS
Vegetable raw material isexposed to various micromycete, among which a large proportion of Fusariumfungi. In the fight against fungal lesions, biological protection methods havebeen widely used, the most promising in this direction is the use of naturalantagonists. Within the framework of this work, the micromycete Fusarium sporotrichioides was isolatedfrom fodder raw materials and pure cultures of microorganisms from naturalbiotopes, the antifungal activity of the isolated microbes was determined bythe methods of counter cultures, agar blocks, modified strokes and delayedantagonism. Active strains were found using any of them, however, the nature ofthe interaction of the antagonist and pathogen fully reveals an analysis ofcomplementary experiments. The presence of fungistatic alimentary antagonism inthe relationships of isolates conventionally designated as SB16, SB17, SB20 andSB23 with the micromycete Fusariumsporotrichioides was determined by the method of counter cultures, thewidth of the fungal mycelia growth zone was below the control by 64.6; 64.3;65.7; 63.0 and 68.8 %, respectively. Fungistatic antibiotic antagonismpossessed isolates SB10 and SB15, therefore, it was characterized by thesynthesis of substances that inhibit the development of mycelium action. Themethod of agar blocks showed the greatest activity of isolates SB1, SB10, SB15,SB16 and SB20, which were, respectively, 1.39 ± 0.04, 1.33 ± 0.17, 2.21 ± 0.19,1.93 ± 0.17 and 2.00 ± 0.11. The largest zone of growth retardation of thepathogen in the formulation of the experiment using the modified stroke methodwas the isolates SB16 and SB17 (9.00 ± 0.35 mm and 13.00 ± 0.35 mm). Thegreatest fungistatic activity revealed by the method of delayed antagonism wasshown by isolates SB1, SB10, SB12, SB15, SB16 and SB20. Thus, the selected isolatesin the future can serve as a basis for obtaining bio fungicides, the mostpromising ones showing an antagonistic effect against micromycete Fusarium sporotrichioides for use asliving cultures were conditionally designated isolates SB17 and SB23, antifungalsubstances isolated SB12, a high antifungal effect during growth and theactivity of metabolites showed isolates SB1, SB10, SB15, SB16 and SB20.
Fusarium sporotrichioides, natural biotopes, isolates, antagonism.
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