of the Urals

Russian Journal of Agricultural Research

The publication is registered by the Ministry of the Russian Federation
for Affairs of the Press, Television and Radio Broadcasting and Mass Communication Media.
Registration certificate: PI number 77-12831 on May 31, 2002
Subscription index in catalog «Russian Press» - 16356
ISSN 1997 - 4868 (Print)

The Journal is included in the list of the leading peer-reviewed scientific journals and publications, which should be published by the main results of theses for the degree of doctor and Ph.D.
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Journal is included in the list of VAK (from 25.09.2017), No. 291

ISSN 2307-0005 (Online)
Key title: Agrarnyj vestnik Urala (Online)
Abbreviated key title: Agrar. vestn. Urala (Online)

Аграрный вестник Урала № 03 (170) 2018

Биология и биотехнологии

И. И. ИДИЯТОВ кандидат биологических наук, старший научный сотрудник, Федеральный центр токсикологической, радиационной и биологической безопасности

Л. Р. ВАЛИУЛЛИН кандидат биологических наук, заведующий сектором Федеральный центр токсикологической, радиационной и биологической безопасности

С. Р. ГАЛЛЯМОВА младший научный сотрудник Федеральный центр токсикологической, радиационной и биологической безопасности

В. В. БИРЮЛЯ кандидат биологических наук, ведущий научный сотрудник Федеральный центр токсикологической, радиационной и биологической безопасности

А. И. НИКИТИН кандидат ветеринарных наук, директор Федеральный центр токсикологической, радиационной и биологической безопасности



Vegetable raw material isexposed to various micromycete, among which a large proportion of Fusariumfungi. In the fight against fungal lesions, biological protection methods havebeen widely used, the most promising in this direction is the use of naturalantagonists. Within the framework of this work, the micromycete Fusarium sporotrichioides was isolatedfrom fodder raw materials and pure cultures of microorganisms from naturalbiotopes, the antifungal activity of the isolated microbes was determined bythe methods of counter cultures, agar blocks, modified strokes and delayedantagonism. Active strains were found using any of them, however, the nature ofthe interaction of the antagonist and pathogen fully reveals an analysis ofcomplementary experiments. The presence of fungistatic alimentary antagonism inthe relationships of isolates conventionally designated as SB16, SB17, SB20 andSB23 with the micromycete Fusariumsporotrichioides was determined by the method of counter cultures, thewidth of the fungal mycelia growth zone was below the control by 64.6; 64.3;65.7; 63.0 and 68.8 %, respectively. Fungistatic antibiotic antagonismpossessed isolates SB10 and SB15, therefore, it was characterized by thesynthesis of substances that inhibit the development of mycelium action. Themethod of agar blocks showed the greatest activity of isolates SB1, SB10, SB15,SB16 and SB20, which were, respectively, 1.39 ± 0.04, 1.33 ± 0.17, 2.21 ± 0.19,1.93 ± 0.17 and 2.00 ± 0.11. The largest zone of growth retardation of thepathogen in the formulation of the experiment using the modified stroke methodwas the isolates SB16 and SB17 (9.00 ± 0.35 mm and 13.00 ± 0.35 mm). Thegreatest fungistatic activity revealed by the method of delayed antagonism wasshown by isolates SB1, SB10, SB12, SB15, SB16 and SB20. Thus, the selected isolatesin the future can serve as a basis for obtaining bio fungicides, the mostpromising ones showing an antagonistic effect against micromycete Fusarium sporotrichioides for use asliving cultures were conditionally designated isolates SB17 and SB23, antifungalsubstances isolated SB12, a high antifungal effect during growth and theactivity of metabolites showed isolates SB1, SB10, SB15, SB16 and SB20.


Fusarium sporotrichioides, natural biotopes, isolates, antagonism.


1. Gagkayeva T. Yu., Gavrilova O. P., Levitin M. M., Novozhilov K. V. Fusarium of grain crops // Supplement to the journal «Plant Protection and Quarantine». 2011. No. 5. 52 p.

2. Sokolova L. M., Egorova A. A., Tereshonkova T. A., Alekseeva K. L. Accelerated method of isolation into pure culture and characteristics of fungi Fusarium, carrots affecting the dining room // Selection and seed-growing of vegetable crops. 2014. No. 45. P. 496–501.

3. Methodological instructions for sanitary and mycological evaluation and improvement of feed quality of February 25, 1985.

4. GOST 13496.6-71 «Mixed fodder: method for the isolation of microscopic fungi».

5. Laboratory workshop on microbiology : training and metodology complex. Minsk : Belarusian State Pedagogical University named after Maxim Tank, 2012. 129 p.

6. Khramtsov A. K., Stefanovich A. I. Mycology: methodical instructions to the special course on the section «Ecology of fungi and mushroom-like organisms» // Minsk : BSU, 2011. 45 p.

7. Asaturova A. M., Dubyaga V. M., Tomashevich N. S., Zharnikova M. D. Selection of promising biological control agents for protection of winter wheat from fusarium pathogens // Scientific journal of KubSAU. 2012. No. 75 (01). URL :

8. Leontievskaya E. A. Structure of epiphytic-saprotrophic bacterial complexes of cereals and vegetable crops : dis. ... cand. biol. sciences. M., 2014. 89 p.

9. Karamova N. S., Nadeeva G. V., Bagaeva Т. V. Methods of research and evaluation of biological damage caused by microorganisms : teaching-methodical manual. Kazan : Kazan University, 2014. 36 p.

10. Idiyatov I. I., Valiullina D. A., Gallyamova S. R. Primary screening of microbial antagonism to microscopic fungi of the genus Aspergillus flavus and Fusarium sporotrichioides // Scientific notes Kazan Bauman State Academy of Veterinary Medicine. 2017. No. 231 (III). P. 90–93.

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