Аграрный вестник Урала № 04 (171) 2018Биология и биотехнологии
COMPARATIVE STUDY OF INDICATORS OF TOXIC ACTIVITY IN THE ALLIUM-TEST
The allium test is one of the most popular methods for assessing the toxic,mutagenic and mitosis-modifying effects of various chemical, physical andbiological factors and is recommended by WHO experts as a standard forcytogenetic monitoring of the environment. In this test, the roots of onionsare used as a test object. In accordance with the procedure of this test, atoxicity test should be performed before the cytogenetic analysis, in which aparameter such as root growth is examined. At the same time, the most accurateand at the same time the most time-consuming method remained, based onmeasuring the average length of roots for the entire sample of bulbs. The workis devoted to the evaluation of the effectiveness of using such a macroscopicindex for detecting the toxicity of mitogens, as the mass of the roots of onions,using the Allium test. When setting up experiments with the standard positivecontrol (copper sulphate solution) for this test, the same significant decreasein both the mean length and the average root mass was shown, while thesensitivity of the root weight was even 3.2 % higher. Similar data wereobtained with a model toxic agent – ethyl alcohol, regardless of the onion variety,duration and temperature of the incubation or type of water from which thesolution was prepared (distilled and bottled). As a result of the research itwas shown that the method of measuring the average mass of bulb roots in theAllium test can be used as the standard parameter of root growth, which is themost simple in application and sensitive.
allium test, toxicity, root growth dynamics, mutagen.
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