Аграрный вестник Урала № 02 (181) 2019Биология и биотехнологии
EFFECT OF THE SOMATIC EXTRACT OF ANISAKIS SIMPLEX L3 TO MICROORGANISMS IN VITRO
In the article, the influence of the somatic extract of Anisakis simplex L3 to the cultures ofmicroorganism cells in vitro is considered. The somatic extract from A. simplex L3 disrupts and depresses theprocess of dividing eukaryotic cells was established earlier. There are veryfew data on the mechanisms of interactions of somatic extracts of helminths andmicroorganisms. Somatic extractanisakids renders a negative effect on microorganisms due to its constituentprotein components and metabolites, it is supposed. Somatic extract from anisakids has anegative effect on microorganisms due to proteins and metabolites in itscomposition, it is assumed. The aim of the study was to study the effect extracton cell cultures of microorganisms diverse in morphology and resistance toenvironmental factors. The extractwas prepared from the larvae of anisakids, the larvae were removed from frozen Micromesistiuspoutassou, then the extract was checked for sterility and harmlessness, theprotein content was determined, then the disks were soaked with somaticextract. For thestudy, daily cultures bacteria were used: micrococci Micrococcus sp., of sticks Escherichiacoli, Proteus vulgaris, Salmonella tiphimurium, and bacilli Bacillus subtilis. Microorganismswere cultured with discs that contained an antigenic extract of anisakids in athermostat at + 37 ° C, after 12 hours, a growth retardation zone was detectedin Micrococcus sp., E. coli and P. vulgaris. The extract had no effect on thegrowth of S.tiphimurium, B. subtilis. Thesterility zone, which is formed temporarily, indicates that the extractcontains biological active components acting bacteriostatically onmicroorganisms. Themechanisms of bacteriostatic action of the somatic extract of Anisakis simplex L3 on bacteria were byassumed.
somatic extract, Anisakis simplex L3, metabolites, bacteria, bacilli, bacteriostatic action.
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