of the Urals

Russian Journal of Agricultural Research

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Registration certificate: PI number 77-12831 on May 31, 2002
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ISSN 1997 - 4868 (Print)

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ISSN 2307-0005 (Online)
Key title: Agrarnyj vestnik Urala (Online)
Abbreviated key title: Agrar. vestn. Urala (Online)

Аграрный вестник Урала № 11 (129) 2014


Ившина И. Б. доктор биологических наук, заведующая лабораторией, Институт экологии и генетики микроорганизмов Уральского отделения РАН, профессор, Пермский государственный национальный исследовательский университет

Куюкина М. С. доктор биологических наук, ведущий научный сотрудник, Институт экологии и генетики микроорганизмов Уральского отделения РАН, профессор Пермский государственный национальный исследовательский университет

Костина Л.В. кандидат биологических наук, старший инженер, Институт экологии и генетики микроорганизмов Уральского отделения РАН

Тищенко А. В. магистрант Пермский государственный национальный исследовательский университет



Development of civilized technologies for heavy metal removals and elimination of heavy metal contamination aftereffects is of current interest. Analogous remediation of metal-contaminated soils is more complex because microbial cells or large exo- polymers do not move freely through the soil. The use of microbially produced surfactants (biosurfactants) is an alternative with potential for remediation of metal-contaminated soils. Rhodococcus-biosurfactants were used to enhance heavy metal mobiliza- tion and extraction from anthropogenically metal-contaminated soils of Perm. Advantages of Rhodococcus biosurfactants, such as ecological safety and increased functional activity compared to synthetic surfactants, are shown. Efficiency of extraction of heavy metal ions using Rhodococcus-biosurfactant (4 g/l) was 21–100 % for the period once their money, the ability of Rhodococcus- biosurfactants to remove heavy metals from soil was 1.5–50.0 times higher than that of a synthetic surfactant of suitable proper- ties, Tween 60. Rhodococcus-biosurfactants increase in mobility and bioavailability of heavy metals for soil microorganisms. Rhodococcus-biosurfactants can be used for decontamination of heavy metal-polluted soils.


anthropogenically polluted soil, heavy metals, bioremediation, Tween 60, Rhodococcus-biosurfactants


1. Vadunina А. F., Korchagina Z. A. Methods for research of physical properties of the soil. М. : Agropromizdat, 1986. 416 p.

2. GN Soil, Decontamination of Populated Areas, Production and Consumer Wastes, Sanitary Soil Protection.

Maximum Allowable Concentrations (MACs) of Chemical Substances in the Soil. M. : Gossanepidnadzor, 2006. 51 p.

3. Dabahov M. V., Dabahova Е. V., Titova V. I. Ecotoxicology and rationing problems. N. Novgorod : VVАGС, 2005. 165 p.

4. Dguvelikyan H. А., Scheglov D. I., Gorbunova N. S. Heavy metal contaminated soils. Ways of control and rationing

of polluted soils. Voronezh : VSU, 2009. 21 p.

5. Ivshina I. B., Kostina L. V., Kamenskikh T. N., Zhuikova V. A., Zhuikova T. V., Bezel’ V. S. Soil Microbiocenosis

as an Indicator of Stability of Meadow Communities in the Environment Polluted with Heavy // Russian Journal of Ecology.

2014. No 2. P. 83–89.

6. Ivshina I. B., Kuyukina M. S., Kostina L. V. Adaptive Mechanisms of Nonspecific Resistance to Heavy Metal Ions

in Alkanotrophic Actinobacteria // Russian Journal of Ecology. 2013. Vol. 44. No 1. P. 123–130.

7. Regional Specialized Collection of Alkanotrophic Microorganisms : Catalog of Strains / ed. by I. B. Ivshina. M. : Nauka,

1994. 163 p.

8. Kostina L. V., Kuyukina M. S., Ivshina I. B. Heavy Metals : Biosorption, Accumulation, and Methods of Recovery. Lambert

Academic Publishing, 2010. 254 p.

9. Kostina L. V., Kuyukina M. S., Ivshina I. B. Treatment proceducers for heavy metal-polluted soils using (bio)surfactants

(review) // Bulletin of Perm University. Biology. 2009. Vol. 10. No 36. P. 95–110.

10. Оrlov D. S. Soil chemistry. M. : MSU, 1992. 400 p.

11. Ayyasamy P. M., Chun S., Lee S. Desorption and dissolution of heavy metals from contaminated soil using Shewanella sp.

(HN-41) amended with various carbon sources // J. Hazard. Mater. 2009. Vol. 161. P. 1095–1102.

12. Christofi N., Ivshina I. B. Microbial surfactants and their use in field studies of soil remediation // J. Appl. Microbiol. 2002.

Vol. 93. P. 915–929.

13. Ivshina I. B., Kuyukina M. S., Philp J. C., Christofi N. Oil desorption from mineral and organic materials using biosurfactant

complexes produced by Rhodococcus species // World J. Microbiol. Biotechnol. 1998. Vol. 14. Р. 711−717.

14. Kuyukina M. S., Ivshina I. B. Rhodococcus Biosurfactants : Biosynthesis, Properties, and Potential Applications // Biology

of Rhodococcus / ed. by H. M. Alvarez. Berlin : Springer-Verlag, 2010. P. 292–313.

15. Kuyukina M. S., Ivshina I. B., Philp J. C., Cristofi N., Dunbar S. A., Ritchkova M. I. Recovery of Rhodococcus biosurfactants

using methyl-tertiary butyl ether extraction // J. Microbiol. Meth. 2001. Vol. 46. P. 149–156.

16. Miller R. M. Biosurfactant-facilitated remediation of metal-contaminated soils // Environ. Health Perspectives. 1995.

Vol. 103. P. 59–62.

17. Wolfaardt G. M., Lawrence J. R., Headley J. V., Robarts R. D. Microbial exopolymers provide a mechanism

for bioaccumulation of contaminants // Microb. Ecol. 1994. Vol. 27. P. 279–291.

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